Callus ≈ Callus
View article: Morphogenic Regulators <i>Baby boom</i> and <i>Wuschel</i> Improve Monocot Transformation
Morphogenic Regulators <i>Baby boom</i> and <i>Wuschel</i> Improve Monocot Transformation Open
While transformation of the major monocot crops is currently possible, the process typically remains confined to one or two genotypes per species, often with poor agronomics, and efficiencies that place these methods beyond the reach of mo…
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A rapid in vitro protocol for callus production in Piper aduncum L. propagation of East Kalimantan supplemented with gradient sucrose solutions Open
The article considers the task of studying how the two processes relate - the destruction of lubricating oil and the wear of the elements of the tribosystem. The paper analyzes one of these indicators - the optical density of the oil, whic…
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Biotechnology Applications of Plant Callus Cultures Open
In ethnopharmacology, and especially in traditional Chinese medicine, medicinal plants have been used for thousands of years. Similarly, agricultural plants have been used throughout the history of mankind. The recent development of the ge…
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High Efficiency In vitro Whole Plant Regeneration via Desiccated Callus in Oryza sativa cv. MTU1010 Open
For most people across the planet, rice is also considered as most beneficial crops in terms of fulfilling every day's energy and nutritional needs. Worldwide food safety is seriously threatened since severe limitations on rice output caus…
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Callus, Dedifferentiation, Totipotency, Somatic Embryogenesis: What These Terms Mean in the Era of Molecular Plant Biology? Open
Recent findings call for the critical overview of some incorrectly used plant cell and tissue culture terminology such as dedifferentiation, callus, totipotency, and somatic embryogenesis. Plant cell and tissue culture methods are efficien…
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Wounding Triggers Callus Formation via Dynamic Hormonal and Transcriptional Changes Open
Wounding is a primary trigger of organ regeneration, but how wound stress reactivates cell proliferation and promotes cellular reprogramming remains elusive. In this study, we combined transcriptome analysis with quantitative hormonal anal…
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Polyploidy Affects Plant Growth and Alters Cell Wall Composition Open
Polyploidization has played a key role in plant breeding and crop improvement. Although its potential to increase biomass yield is well described, the effect of polyploidization on biomass composition has largely remained unexplored. Here,…
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WIND1 Promotes Shoot Regeneration through Transcriptional Activation of <i>ENHANCER OF SHOOT REGENERATION1</i> in Arabidopsis Open
Many plant species display remarkable developmental plasticity and regenerate new organs after injury. Local signals produced by wounding are thought to trigger organ regeneration but molecular mechanisms underlying this control remain lar…
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Developmentally Engineered Callus Organoid Bioassemblies Exhibit Predictive In Vivo Long Bone Healing Open
Clinical translation of cell‐based products is hampered by their limited predictive in vivo performance. To overcome this hurdle, engineering strategies advocate to fabricate tissue products through processes that mimic development and reg…
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Silver nanoparticles elicited <i>in vitro</i> callus cultures for accumulation of biomass and secondary metabolites in <i>Caralluma tuberculata</i> Open
Elicited plant in vitro cultures are gaining more interest worldwide for their potential in the uniform production of industrially important secondary metabolites. In the present study, different ratios of silver nanoparticles (AgNPs) and …
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Impact of application of zinc oxide nanoparticles on callus induction, plant regeneration, element content and antioxidant enzyme activity in tomato (Solanum lycopersicum Mill.) under salt stress Open
The properties of nanomaterials and their potential applications have been given considerable attention by researchers in various fields, especially agricultural biotechnology. However, not much has been done to evaluate the role or effect…
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Lignin biosynthesis genes play critical roles in the adaptation of <i>Arabidopsis</i> plants to high-salt stress Open
Salinity is a major abiotic stressor that limits the growth, development, and reproduction of plants. Our previous metabolic analysis of high salt-adapted callus suspension cell cultures from Arabidopsis roots indicated that physical reinf…
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The WOX11–LBD16 Pathway Promotes Pluripotency Acquisition in Callus Cells During De Novo Shoot Regeneration in Tissue Culture Open
De novo shoot regeneration in tissue culture undergoes at least two phases. Explants are first cultured on auxin-rich callus-inducing medium (CIM) to produce a group of pluripotent cells termed callus; the callus is then transferred to cyt…
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Very-long-chain fatty acids restrict regeneration capacity by confining pericycle competence for callus formation in <i>Arabidopsis</i> Open
Significance Callus induction is an initial step for typical plant in vitro regeneration, and recent studies show that auxin-induced callus formation in multiple organs occurs from the pericycle or pericycle-like cells via a root developme…
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Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis Open
Body regeneration through formation of new organs is a major question in developmental biology. We investigated de novo root formation using whole leaves of Arabidopsis (Arabidopsis thaliana). Our results show that local cytokinin biosynth…
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Spatial transcriptomics reveals light-induced chlorenchyma cells involved in promoting shoot regeneration in tomato callus Open
Callus is a reprogrammed cell mass involved in plant regeneration and gene transformation in crop engineering. Pluripotent callus cells develop into fertile shoots through shoot regeneration. The molecular basis of the shoot regeneration p…
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miR156-SPL modules regulate induction of somatic embryogenesis in citrus callus Open
miR156 is a highly conserved plant miRNA and has been extensively studied because of its versatile roles in plant development. Here, we report a novel role of miR156 in regulating somatic embryogenesis (SE) in citrus, one of the most widel…
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Oxidative damage, antioxidant mechanism and gene expression in tomato responding to salinity stress under in vitro conditions and application of iron and zinc oxide nanoparticles on callus induction and plant regeneration Open
Background Salinity is one of the most challenging abiotic stresses restricting the growth of plants. In vitro screening will increase the efficiency and speed of salinity tolerant genotypes identifications. The response of four tomato cul…
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Antimicrobial and In Vitro Cytotoxic Efficacy of Biogenic Silver Nanoparticles (Ag-NPs) Fabricated by Callus Extract of Solanum incanum L. Open
The in vitro callus induction of Solanum incanum L. was executed on MS medium supplemented with different concentrations of auxin and cytokinin utilizing petioles and explants of leaves. The highest significant fresh weights from petioles …
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Efficient Callus Induction and Regeneration in Selected Indica Rice Open
An efficient callus induction and in vitro regeneration were developed using plant growth regulators, carbon sources, and basal media for three selected Malaysian wetland rice varieties (MR220, MR220-CL2, and MR232) and one upland variety …
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Motion Predicts Clinical Callus Formation Open
Background: Mechanotransduction is theorized to influence fracture-healing, but optimal fracture-site motion is poorly defined. We hypothesized that three-dimensional (3-D) fracture-site motion as estimated by finite element (FE) analysis …
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Genetic Dissection of Maize Embryonic Callus Regenerative Capacity Using Multi-Locus Genome-Wide Association Studies Open
The regenerative capacity of the embryonic callus, a complex quantitative trait, is one of the main limiting factors for maize transformation. This trait was decomposed into five traits, namely, green callus rate (GCR), callus differentiat…
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Insights into antioxidant activities and anti-skin-aging potential of callus extract from Centella asiatica (L.) Open
Formation of oxidative stress in dermal fibroblasts plays crucial roles in aging processes of skin. The use of phytochemicals that can promote capacity of fibroblasts to combat oxidative stress is an attractive strategy to prevent skin agi…
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Auxin Control of Root Organogenesis from Callus in Tissue Culture Open
OPINION article Front. Plant Sci., 08 August 2017Sec. Plant Physiology Volume 8 - 2017 | https://doi.org/10.3389/fpls.2017.01385
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ROOTING OF Ficus enormis MINI-CUTTINGS WITH DIFFERENT LENGTHS Open
Ficus enormis is a native species with great ecological potential, especially for ecosystems restoration. The present study aimed to evaluate the rooting, root and vegetative vigor of Ficus enormis mini-cuttings and to determine its best l…
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Tissue Culture of Oil Palm: Finding the Balance Between Mass Propagation and Somaclonal Variation Open
The oil palm (Elaeis guineensis Jacq.) is typically propagated in vitro by indirect somatic embryogenesis, a process in which somatic cells of an explant of choice are, via an intermediate phase of callus growth, induced to differentiate i…
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Gamma irradiation of medicinally important plants and the enhancement of secondary metabolite production Open
Purpose: The profitable production of some important plant-based secondary metabolites (ginsenosides, saponins, camptothecin, shikonins etc.) in vitro by gamma irradiation is a current area of interest. We reviewed different types of secon…
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Dynamic Stabilization with Active Locking Plates Delivers Faster, Stronger, and More Symmetric Fracture-Healing Open
Background: Axial dynamization of fractures can promote healing, and overly stiff fixation can suppress healing. A novel technology, termed active plating, provides controlled axial dynamization by the elastic suspension of locking holes w…
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Age-associated callus senescent cells produce TGF-β1 that inhibits fracture healing in aged mice Open
Cellular senescence plays an important role in human diseases, including osteoporosis and osteoarthritis. Senescent cells (SCs) produce the senescence-associated secretory phenotype to affect the function of neighboring cells and SCs thems…
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Chemometric analysis reveals links in the formation of fragrant bio-molecules during agarwood (Aquilaria malaccensis) and fungal interactions Open
Fragrant agarwood, arguably the costliest wood in the world, is formed by plant-fungal interactions in Aquilaria spp. However, very little is known about this fragrant outcome of interaction. Therefore, mimicking the ancient traditions of …