Flow cytometry ≈ Flow cytometry
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Third-generation <i>in situ</i> hybridization chain reaction: multiplexed, quantitative, sensitive, versatile, robust Open
In situ hybridization based on the mechanism of the hybridization chain reaction (HCR) has addressed multi-decade challenges that impeded imaging of mRNA expression in diverse organisms, offering a unique combination of multiplexing, quant…
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Simultaneous enumeration of cancer and immune cell types from bulk tumor gene expression data Open
Immune cells infiltrating tumors can have important impact on tumor progression and response to therapy. We present an efficient algorithm to simultaneously estimate the fraction of cancer and immune cell types from bulk tumor gene express…
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Characteristics of Peripheral Lymphocyte Subset Alteration in COVID-19 Pneumonia Open
Background In December 2019, novel coronavirus (SARS-CoV-2) pneumonia (COVID-19) was reported in Wuhan and has since rapidly spread throughout China. We aimed to clarify the characteristics and clinical significance of peripheral lymphocyt…
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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition) Open
These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors…
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Immunophenotyping of COVID-19 and influenza highlights the role of type I interferons in development of severe COVID-19 Open
Single-cell RNA sequencing of blood immune cells reveals type I interferon–associated hyper-inflammation in severe COVID-19.
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Flow cytometry: basic principles and applications Open
Flow cytometry is a sophisticated instrument measuring multiple physical characteristics of a single cell such as size and granularity simultaneously as the cell flows in suspension through a measuring device. Its working depends on the li…
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Gene expression markers of Tumor Infiltrating Leukocytes Open
Due to their concise gene set, computational simplicity and utility in tumor samples, these cell type gene signatures may be useful in future discovery research and clinical trials to understand how tumors and therapeutic intervention shap…
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Single-Cell Analysis Reveals Fibroblast Clusters Linked to Immunotherapy Resistance in Cancer Open
A subset of cancer-associated fibroblasts (FAP+/CAF-S1) mediates immunosuppression in breast cancers, but its heterogeneity and its impact on immunotherapy response remain unknown. Here, we identify 8 CAF-S1 clusters by analyzing more than…
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Flow Cytometry: An Overview Open
Flow cytometry is a technology that provides rapid multi‐parametric analysis of single cells in solution. Flow cytometers utilize lasers as light sources to produce both scattered and fluorescent light signals that are read by detectors su…
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Carcinoma-associated fibroblasts promote the stemness and chemoresistance of colorectal cancer by transferring exosomal lncRNA H19 Open
Long non-coding RNAs (lncRNAs) are involved in the pathology of various tumors, including colorectal cancer (CRC). The crosstalk between carcinoma- associated fibroblasts (CAFs) and cancer cells in the tumor microenvironment promotes tumor…
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Redefining Tumor-Associated Macrophage Subpopulations and Functions in the Tumor Microenvironment Open
The immunosuppressive status of the tumor microenvironment (TME) remains poorly defined due to a lack of understanding regarding the function of tumor-associated macrophages (TAMs), which are abundant in the TME. TAMs are crucial drivers o…
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Guidelines for cell viability assays Open
Recently, the interest in the application of cell viability assays has been increasing in various fields. Cell viability assays may be broadly classified as (a) dye exclusion assays, (b) colorimetric assays, (c) fluorometric assays, (d) lu…
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Quality and efficiency assessment of six extracellular vesicle isolation methods by nano‐flow cytometry Open
Extracellular vesicles (EVs) have sparked tremendous interest owing to their prominent potential in diagnostics and therapeutics. Isolation of EVs from complex biological fluids with high purity is essential to the accurate analysis of EV …
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Clinical Significance of PD-L1+ Exosomes in Plasma of Head and Neck Cancer Patients Open
Purpose: The microenvironment of head and neck squamous cell carcinomas (HNSCC) is highly immunosuppressive. HNSCCs expressing elevated levels of PD-L1 have especially poor outcome. Exosomes that carry PD-L1 and suppress T-cell functions h…
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Guidelines for the use of flow cytometry and cell sorting in immunological studies <sup>*</sup> Open
The marriage between immunology and cytometry is one of the most stable and productive in the recent history of science. A rapid search in PubMed shows that, as of July 2017, using “flow cytometry immunology” as a search term yields more t…
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Atlas of the Immune Cell Repertoire in Mouse Atherosclerosis Defined by Single-Cell RNA-Sequencing and Mass Cytometry Open
Rationale: Atherosclerosis is a chronic inflammatory disease that is driven by the interplay of pro- and anti-inflammatory leukocytes in the aorta. Yet, the phenotypic and transcriptional diversity of aortic leukocytes is poorly understood…
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Tumor immune profiling predicts response to anti–PD-1 therapy in human melanoma Open
This work was funded by a generous gift provided by Inga-Lill and David Amoroso as well as a generous gift provided by Stephen Juelsgaard and Lori Cook.
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Tumour-activated neutrophils in gastric cancer foster immune suppression and disease progression through GM-CSF-PD-L1 pathway Open
Objective Neutrophils are prominent components of solid tumours and exhibit distinct phenotypes in different tumour microenvironments. However, the nature, regulation, function and clinical relevance of neutrophils in human gastric cancer …
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Natural killer cell immunotypes related to COVID-19 disease severity Open
The NK cell activation landscape in acute SARS-CoV-2 infection is associated with COVID-19 disease severity.
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Biology and relevance of human acute myeloid leukemia stem cells Open
Evidence of human acute myeloid leukemia stem cells (AML LSCs) was first reported nearly 2 decades ago through the identification of rare subpopulations of engrafting cells in xenotransplantation assays. These AML LSCs were shown to reside…
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MIFlowCyt‐EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments Open
Extracellular vesicles (EVs) are small, heterogeneous and difficult to measure. Flow cytometry (FC) is a key technology for the measurement of individual particles, but its application to the analysis of EVs and other submicron particles h…
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Exosomal circPACRGL promotes progression of colorectal cancer via the miR-142-3p/miR-506-3p- TGF-β1 axis Open
Background Colorectal cancer (CRC) is the leading cause of cancer-related death worldwide. Exosome shave emerged as crucial regulators of intercellular communication and that abundant Circular RNAs (circRNAs) are enriched within exosomes. …
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Exosomes Derived From Natural Killer Cells Exert Therapeutic Effect in Melanoma Open
Objective: Exosomes are nanovesicles that are released from normal and tumor cells and are detectable in cell culture supernatant and human biological fluids. Although previous studies have explored exosomes released from cancer cells, lit…
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Glioblastoma-infiltrated innate immune cells resemble M0 macrophage phenotype Open
Glioblastomas are highly infiltrated by diverse immune cells, including microglia, macrophages, and myeloid-derived suppressor cells (MDSCs). Understanding the mechanisms by which glioblastoma-associated myeloid cells (GAMs) undergo metamo…
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Development and validation of a TP53-associated immune prognostic model for hepatocellular carcinoma Open
TP53 mutation is strongly related to the immune microenvironment in HCC. Our IPM, which is sensitive to TP53 mutation status, may have important implications for identifying subgroups of HCC patients with low or high risk of unfavourable s…
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Transcriptome Analysis Reveals Nonfoamy Rather Than Foamy Plaque Macrophages Are Proinflammatory in Atherosclerotic Murine Models Open
Rationale: Monocyte infiltration into the subintimal space and its intracellular lipid accumulation are the most prominent features of atherosclerosis. To understand the pathophysiology of atherosclerotic disease, we need to understand the…
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Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) Open
The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine im…
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Neutrophils dominate the immune cell composition in non-small cell lung cancer Open
The response rate to immune checkpoint inhibitor therapy for non-small-cell lung cancer (NSCLC) is just 20%. To improve this figure, several early phase clinical trials combining novel immunotherapeutics with immune checkpoint blockade hav…
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Standardized flow cytometry for highly sensitive MRD measurements in B-cell acute lymphoblastic leukemia Open
Key Points Standardized flow cytometry allows highly sensitive MRD measurements in virtually all BCP-ALL patients. If sufficient cells are measured (>4 million), flow cytometric MRD analysis is at least as sensitive as current PCR-based MR…
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Antibody–Fc/FcR Interaction on Macrophages as a Mechanism for Hyperprogressive Disease in Non–small Cell Lung Cancer Subsequent to PD-1/PD-L1 Blockade Open
Purpose: Hyperprogression (HP), a paradoxical boost in tumor growth, was described in a subset of patients treated with immune checkpoint inhibitors (ICI). Neither clinicopathologic features nor biological mechanisms associated with HP hav…