Lipid bilayer ≈ Lipid bilayer
View article: Membrane Electroporation and Electropermeabilization: Mechanisms and Models
Membrane Electroporation and Electropermeabilization: Mechanisms and Models Open
Exposure of biological cells to high-voltage, short-duration electric pulses causes a transient increase in their plasma membrane permeability, allowing transmembrane transport of otherwise impermeant molecules. In recent years, large step…
View article
Antimicrobial Peptides: Mechanisms of Action and Resistance Open
More than 40 antimicrobial peptides and proteins (AMPs) are expressed in the oral cavity. These AMPs have been organized into 6 functional groups, 1 of which, cationic AMPs, has received extensive attention in recent years for their promis…
View article
Stimuli‐responsive liposomes for drug delivery Open
The ultimate goal of drug delivery is to increase the bioavailability and reduce the toxic side effects of the active pharmaceutical ingredient ( API ) by releasing them at a specific site of action. In the case of antitumor therapy, assoc…
View article
An emerging focus on lipids in extracellular vesicles Open
Extracellular vesicles contain a lipid bilayer membrane that protects the encapsulated material, such as proteins, nucleic acids, lipids and metabolites, from the extracellular environment. These vesicles are released from cells via differ…
View article
Cubosomes: The Next Generation of Smart Lipid Nanoparticles? Open
Cubosomes are highly stable nanoparticles formed from the lipid cubic phase and stabilized by a polymer based outer corona. Bicontinuous lipid cubic phases consist of a single lipid bilayer that forms a continuous periodic membrane lattice…
View article
Daptomycin inhibits cell envelope synthesis by interfering with fluid membrane microdomains Open
Significance To date, simple membrane pore formation resulting in cytoplasmic leakage is the prevailing model for how membrane-active antibiotics kill bacteria and also is one of the main explanations for the activity of the membrane-bindi…
View article
Molecular machines open cell membranes Open
Beyond the more common chemical delivery strategies, several physical techniques are used to open the lipid bilayers of cellular membranes. These include using electric and magnetic fields, temperature, ultrasound or light to introduce com…
View article
Oral delivery of therapeutic peptides and proteins: Technology landscape of lipid-based nanocarriers Open
The oral administration of therapeutic peptides and proteins is favoured from a patient and commercial point of view. In order to reach the systemic circulation after oral administration, these drugs have to overcome numerous barriers incl…
View article
Nanoplasmonic Sensor Detects Preferential Binding of IRSp53 to Negative Membrane Curvature Open
Biosensors based on plasmonic nanostructures are widely used in various applications and benefit from numerous operational advantages. One type of application where nanostructured sensors provide unique value in comparison with, for instan…
View article
Circular dichroism spectroscopy of membrane proteins Open
Circular dichroism spectra of helical bundle (red), beta barrel (blue), and mixed helical/sheet/unordered (green) membrane proteins.
View article
Membrane curvature in cell biology: An integration of molecular mechanisms Open
Curving biological membranes establishes the complex architecture of the cell and mediates membrane traffic to control flux through subcellular compartments. Common molecular mechanisms for bending membranes are evident in different cell b…
View article
Lipid21: Complex Lipid Membrane Simulations with AMBER Open
We extend the modular AMBER lipid force field to include anionic lipids, polyunsaturated fatty acid (PUFA) lipids, and sphingomyelin, allowing the simulation of realistic cell membrane lipid compositions, including raft-like domains. Head …
View article
Outer Membrane Biogenesis Open
The hallmark of gram-negative bacteria and organelles such as mitochondria and chloroplasts is the presence of an outer membrane. In bacteria such as Escherichia coli, the outer membrane is a unique asymmetric lipid bilayer with lipopolysa…
View article
Spatial arrangement of proteins in planar and curved membranes by <span>PPM</span> 3.0 Open
Cellular protrusions, invaginations, and many intracellular organelles have strongly curved membrane regions. Transmembrane and peripheral membrane proteins that induce, sense, or stabilize such regions cannot be properly fitted into a sin…
View article
Permeability and Stability of Lipid Bilayers Open
I. INTRODUCTION The lipid bilayers of natural membranes generally exist in a fluid state which occurs above the gel-toliquid-crystalline phase transition temperature.1 Knowledge of the structure of such “fluid” bilayers, which is of obviou…
View article
Bacterial Membranes: Structure, Domains, and Function Open
The bacterial cytoplasmic membrane is composed of roughly equal proportions of lipids and proteins. The main lipid components are phospholipids, which vary in acyl chain length, saturation, and branching and carry head groups that vary in …
View article
New tricks for old dogs: improving the accuracy of biomolecular force fields by pair-specific corrections to non-bonded interactions Open
Recent advances in parallel computing have pushed all-atom molecular dynamics simulations into an untested territory. This article reviews the applications of the NBFIX approach for testing and improving molecular dynamics force fields and…
View article
ROS induced lipid peroxidation and their role in ferroptosis Open
Reactive oxygen species (ROS) play a crucial part in the process of cell death, including apoptosis, autophagy, and ferroptosis. ROS involves in the oxidation of lipids and generate 4-hydroxynonenal and other compounds associated with it. …
View article
Drug Delivery via Cell Membrane Fusion Using Lipopeptide Modified Liposomes Open
Efficient delivery of drugs to living cells is still a major challenge. Currently, most methods rely on the endocytotic pathway resulting in low delivery efficiency due to limited endosomal escape and/or degradation in lysosomes. Here, we …
View article
Quality by Design-Driven Zeta Potential Optimisation Study of Liposomes with Charge Imparting Membrane Additives Open
Liposomal formulations, as versatile nanocarrier systems suitable for targeted delivery, have a highly focused role in the therapy development of unmet clinical needs and diagnostic imaging techniques. Formulating nanomedicine with suitabl…
View article
Solubilization of Membrane Proteins into Functional Lipid‐Bilayer Nanodiscs Using a Diisobutylene/Maleic Acid Copolymer Open
Once removed from their natural environment, membrane proteins depend on membrane‐mimetic systems to retain their native structures and functions. To this end, lipid‐bilayer nanodiscs that are bounded by scaffold proteins or amphiphilic po…
View article
Dimers of mitochondrial ATP synthase induce membrane curvature and self-assemble into rows Open
Significance The ATP synthase in the inner membrane of mitochondria generates most of the ATP that enables higher organisms to live. The inner membrane forms deep invaginations called cristae. Mitochondrial ATP synthases are dimeric comple…
View article
Protein Crowding in Lipid Bilayers Gives Rise to Non-Gaussian Anomalous Lateral Diffusion of Phospholipids and Proteins Open
Biomembranes are exceptionally crowded with proteins with typical protein-to-lipid ratios being around 1:50 - 1:100. Protein crowding has a decisive role in lateral membrane dynamics as shown by recent experimental and computational studie…
View article
Current Strategies for Exosome Cargo Loading and Targeting Delivery Open
Extracellular vesicles (EVs) such as ectosomes and exosomes have gained attention as promising natural carriers for drug delivery. Exosomes, which range from 30 to 100 nm in diameter, possess a lipid bilayer and are secreted by various cel…
View article
Structure of the human TRPM4 ion channel in a lipid nanodisc Open
Architecture of the TRPM subfamily Transient receptor potential melastatin (TRPM) ion channels constitute the largest TRP subfamily and are involved in many physiological processes. TRPM8 is the primary cold and menthol sensor, and TRPM4 i…
View article
Aβ42 assembles into specific β-barrel pore-forming oligomers in membrane-mimicking environments Open
Significance Numerous reports indicate that amyloid-β peptide (Aβ) oligomers, considered the pathogenic molecular form of Aβ in Alzheimer´s disease (AD), exert their neurotoxicity within the membrane. Therefore, it is critical to character…
View article
Characterization of Lipid–Protein Interactions and Lipid-Mediated Modulation of Membrane Protein Function through Molecular Simulation Open
The cellular membrane constitutes one of the most fundamental compartments of a living cell, where key processes such as selective transport of material and exchange of information between the cell and its environment are mediated by prote…
View article
Ferroptotic pores induce Ca2+ fluxes and ESCRT-III activation to modulate cell death kinetics Open
Ferroptosis is an iron-dependent form of regulated necrosis associated with lipid peroxidation. Despite its key role in the inflammatory outcome of ferroptosis, little is known about the molecular events leading to the disruption of the pl…
View article
Predicting a Drug’s Membrane Permeability: A Computational Model Validated With <i>in Vitro</i> Permeability Assay Data Open
Membrane permeability is a key property to consider during the drug design process, and particularly vital when dealing with small molecules that have intracellular targets as their efficacy highly depends on their ability to cross the mem…
View article
Monodisperse Uni- and Multicompartment Liposomes Open
Liposomes are self-assembled phospholipid vesicles with great potential in fields ranging from targeted drug delivery to artificial cells. The formation of liposomes using microfluidic techniques has seen considerable progress, but the lip…