Amino acid analysis
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Protein Analysis Open
Protein content analysis of foods and ingredients is important for a variety of reasons. This chapter covers the instrumentation, principles, procedures, advantages, disadvantages, and applications of various protein analysis methods that …
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Chemical profiling of mycosporine‐like amino acids in twenty‐three red algal species Open
Rhodophyta produce a variety of chemically different mycosporine‐like amino acids (MAAs), compounds that are known as some of the strongest ultraviolet (UV) absorbing molecules in nature. Accordingly, they primarily act as photoprotectants…
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Amino Acid Profiles and Nutritional Evaluation of Fresh Sweet–Waxy Corn from Three Different Regions of China Open
This study conducted a comparative analysis of the amino acid compositions of Chinese Huangnuo 9 fresh sweet–waxy corn from three different provinces in China—Inner Mongolia, Jilin, and Heilongjiang Province. Moreover, we established a nut…
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Biochemical analysis of collagens from the bone of lizardfish (<i>Saurida tumbil</i> Bloch, 1795) extracted with different acids Open
Background Lizardfish ( Saurida tumbil Bloch, 1795) bone is a fish by-product generated during industrial surimi processing. This by-product is an important source of collagen production since the use of terrestrial animal-based collagens …
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Amino acid profile and protein quality in tuber and leaf of <i>Coccnia abyssinica</i> (Lam.) (Cogn.) accessions of Ethiopia Open
The protein content and amino acid profile of Anchote ( Coccinia abyssinica ) leaves and tubers were determined from ten different accessions taken from Debre Zeit Agricultural Research Center, Ethiopia. Crude protein content was determine…
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Chemical Composition and Amino Acid Content in Different Genotypes of Wheat Flour Open
The purpose of this study was to evaluate the chemical parameters and amino acid content in 10 genotypes of soft Albanian wheat organically grown and collected during the summer seasons of 2014. The thin layer chromatography method was use…
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Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic Measurement Open
Occurrence of d-amino acids in living organisms is a useful indicator of various changes, diseases, or disorders. Determination of amino acid enantiomers, namely the enantiomeric ratio of amino acids or excess of certain d-amino acids, rep…
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Collagen isolation with acid soluble method from the skin of Red Snapper (<i>lutjanus</i> sp.) Open
Collagen is an extracellular matrix connective tissue that account for about 30% of the total protein. Red snapper skin has the potential as an alternative source of collagen that can increase the added value of food and fishery industrial…
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Swim Bladder of Farmed Totoaba macdonaldi: A Source of Value-Added Collagen Open
Finding strategies to use the swim bladder of farmed totoaba (Totoaba macdonaldi) is of the utmost need to reduce waste. Fish swim bladders are rich in collagen; hence, extracting collagen is a promising alternative with benefits for aquac…
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Development of Precolumn Derivatization–LC/MS for Amino-Acid-Focused Metabolomics Open
Focused metabolomics facilitates the understanding of the current phenotype of living organisms, involves highly practical methods for investigation, and allows for the acquisition of highly quantitative data for important endogenous metab…
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Analysis of balenine in muscle extract of opah <i>Lampris guttatus</i> with automatic amino acid analyzer Open
既報の6M塩酸中110℃で24時間加水分解後,アミノ酸自動分析計による3-メチルヒスチジン(3-Mehis)定量値をバレニン(Bal)量とする分析法を再検討した。3-Mehis定量値のばらつきが最小となる加水分解時間は3時間,3-Mehis/Bal比が安定し両者の間に高い直線的相関が得られるBal濃度は0.05-10 μmol/mLでBalからの3-Mehis生成率は89%だった。本法でアカマンボウ筋肉エキスを分析したところヒゲクジラ類を上回る高濃度のBalが含まれることが…
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Amino Acid Profiling and SDS-PAGE Analysis of Protein Isolates Obtained from Nonconventional Sources Open
Proteins play an imperative role in enhancing the nutritional status of the human body. The present study was designed to determine the molecular weight of protein isolates prepared from defatted oilseeds, i.e., sesame, flaxseed, and canol…
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Determining amino acid requirements in humans Open
Amino acids form the building blocks of body protein. Dietary protein sources provide the amino acids needed, but protein sources vary widely in amio acid composition. To ensure humans can meet body demands for amino acids, amino acid inta…
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Determination of the Protein and Amino Acid Content of Fruit, Vegetables and Starchy Roots for Use in Inherited Metabolic Disorders Open
Amino acid (AA)-related inherited metabolic disorders (IMDs) and urea cycle disorders (UCDs) require strict dietary management including foods low in protein such as fruits, vegetables and starchy roots. Despite this recommendation, there …
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A Practical Method for Amino Acid Analysis by LC-MS Using Precolumn Derivatization with Urea Open
Amino acid (AA) analysis is important in biochemistry, food science, and clinical medicine. However, due to intrinsic limitations, AAs usually require derivatization to improve their separation and determination. Here, we present a liquid …
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Dynamic Variation of Amino Acid Contents and Identification of Sterols in Xinyang Mao Jian Green Tea Open
As important biomolecules in Camellia sinensis L., amino acids (AAs) are considered to contribute to the overall green tea sensory quality and undergo dynamic changes during growth. However, limited by analytical capacity, detailed AAs com…
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An electronic circular dichroism spectroscopy method for the quantification of L- and D-amino acids in enantiomeric mixtures Open
Rapid quantitative analysis of single chiral amino acid (aa) was achieved using circular dichroism (CD) with data analysis by standard calibration curve. The absolute concentrations of D- and L-aas in enantiomeric mixtures were determined …
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Quantitative Amino Acid Analysis in Insulin and C-Peptide Assays Open
To the Editor: Standardization of peptide assays is challenging. Most commercial assays are standardized against WHO reference materials. Unfortunately, many of these materials are provided ( a ) with values that are not gravimetrically tr…
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Analysis of amino acids in cell culture supernatant using capillary electrophoresis with contactless conductivity detection Open
CE‐C 4 D methods for the analysis of amino acids (AAs) are presented. Combining the results from two methods with acetic acid and cyclodextrin‐based BGEs, 20 proteinogenic AAs could be analyzed using CE. CE‐C 4 D was also, for the first ti…
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Amino Acid Profiling with Chemometric Analysis as a Feasible Tool for the Discrimination of Marine-Derived Peptide Powders Open
Marine-derived peptide powders have suffered from adulteration via the substitution of lower-price peptides or the addition of adulterants in the market. This study aims to establish an effective approach for the discrimination and detecti…
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UHPLC–electrospray ionization–mass spectrometric analysis of brain cell-specific glucogenic and neurotransmitter amino acid content Open
Astrocyte glycogen, the primary energy reserve in brain, undergoes continuous remodeling by glucose passage through the glycogen shunt prior to conversion to the oxidizable energy fuel l -lactate. Glucogenic amino acids (GAAs) are a potent…
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Complementary and efficient methods for di- and tri-peptide analysis and amino acid quantification from simulated gastrointestinal digestion of collagen hydrolysate Open
Collagen hydrolysates (CHs) are composed of bioactive peptides (BAPs) and amino acids (AAs), which contribute to their health enhancing properties. Post digestion profiling of CHs typically evaluates either BAP or AA content in blood but n…
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Free Amino Acid Composition of Korean Spinach (Spinacia oleracea) Cultivars as Influenced by Different Harvesting Time Open
BACKGROUND:There is lack of comprehensive compositional data of the amino acid profile of spinach with regard to different cultivars.A more detailed knowledge in this aspect will be of benefit in the future selection of spinach genotypes w…
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Intact Protein Analysis at 21 Tesla and X-Ray Crystallography Define Structural Differences in Single Amino Acid Variants of Human Mitochondrial Branched-Chain Amino Acid Aminotransferase 2 (BCAT2) Open
Structural technologies are an essential component in the design of precision therapeutics. Precision medicine entails the development of therapeutics directed toward a designated target protein, with the goal to deliver the right drug to …
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Ion chromatography and ion chromatography / mass spectrometry as a complementary analysis technique for amino acid analysis in food, a review Open
Background: Amino acids are the building blocks of life. Nine of the 20 common amino acids need to be consumed from food, as they are not created within the body. Current amino acid analyser methodology requires either derivatisation which…
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Extraction and characterization of acid soluble collagen (ASC) from airbladder of striped cat fish (Pangasius hypophthalmus) Open
The aim of the present study was to extract high pure acid soluble collagen (ASC) from the airbladder of striped cat fish (Pangasius hypophthalmus). ASC was extracted and its physico-chemical characterization was carried out to confirm the…
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Extraction and Characterization of Collagen from Sand Sea Cucumber (Holothuria scabra) Open
Sand sea cucumber (Holothuria scabra) is an aquatic product that belongs to Echinodermata, a habitant in almost all Indonesian seas. The main component of the sea cucumber is protein, one of which is collagen. This study aimed to extract a…
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Comparison of physical and chemical characteristics of collagen from the skin of cod (Gadus macrocephaius) Open
Acid and pepsin-soluble collagen (ASC and PSC, respectively) were extracted from cod (Gadus macrocephaius) skin, and yields of 37.36 and 55.96% were obtained for ASC and PSC, respectively. The total yield of ASC and PSC was 93.92%, based o…
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Options for substantiating protein content claims for conventional foods Open
In Canada and the United States, front-of-package protein content claims require data to support the quality of the protein. In general, protein quality reflects the product of the amino acid composition of the food protein relative to hum…
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Development of Certified Reference Material for Amino Acids in Dried Blood Spots and Accuracy Assessment of Disc Sampling Open
To achieve the measurement reliability of amino acids used as diagnostic markers in clinical fields, establishing a reference measurement system is required, in which certified reference materials (CRMs) are an essential step in the hierar…