RELATIONSHIP BETWEEN REACTIVE OXYGEN SPECIES, VITAMIN E, CATALASE AND CHLAMYDIA TRACHOMATIS IN SERUM OF INFERTILE FEMALE Article Swipe
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· 2025
· Open Access
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· DOI: https://doi.org/10.5281/zenodo.17539302
Background: The failure in female reproductive system could be cause infertility according to different factors such as sperm isoantobody, zona pellucid antibody, ovarian antibody, Chlamydia trachomatis infection, hormonal imbalance and even between oxygen radical and antioxygen radical. These factors might deteriorate female infertility by different mechanisms. Material and Methods: retrospective case-control study was performed on 105 infertile Jordanian female including 75(71.4%) with primary infertility, and 30(28.6%) with secondary infertility. Thirty healthy women were used as a control. Enzyme labeled and Biotin labeled antibody kits were used in the serum and cervical secretion for determination. Polymerase Chain Reaction (PCR) assay was used to assess the Chlamydia trachomatis in the cervical secretion. Finally, the mean level of Reactive Oxygen Species (ROS) and anti ROS including Vitamin E, and Catalase (CAT) enzyme were estimated in the cervical secretion Results: Serum samples obtained from fertile and infertile females were analyzed for the presence of C. trachomatis specific IgG. 5 (4.76%) of tubal damage infertile females showed the presence of Chlamydial antibody with no detectable level in the serum of fertile females. To confirm the presence of C. trachomatis, PCR was used to identify Chlamydia DNA in the cervical secretion. Only positive serum samples revealed clear, sharp bands of amplified Chlamydial DNA with 241bp. The presence of reactive oxygen species (ROS) within the cervical secretion cancontribute to female infertility if dose not controlled by anti-oxidants. The results revealed no statistical difference in the mean level of ROS, and anti ROS among infertility cases and control. However, the mean antioxidant capacity (vitamin E and catalase) among infertile females with combine infertility problems. Conclusion: We concluded that isoimmunity to sperm and autoimmunity to ova antigens accompanied by elevation in the reactive oxygen species play an ambiguous role in fertility impairment.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.5281/zenodo.17539302
- OA Status
- green
- OpenAlex ID
- https://openalex.org/W7104284762