CD95L concatemers highlight different stoichiometries of CD95‐mediated apoptotic and nonapoptotic pathways Article Swipe

PDF

Related Concepts

jurkat cells random hexamer apoptosis concatemer biology cell biology fas receptor dimer stoichiometry programmed cell death biophysics molecular biology biochemistry chemistry t cell immunology immune system gene genome organic chemistry

Eden Lebrault , Christelle Oblet , Keerthi Kurma , Nicolas Levoin , Robin Jeannet , Mickaël Jean , Pierre Vacher , Patrick Legembre ·

YOU? · · 2023 · Open Access · · DOI: https://doi.org/10.1002/eji.202350626 · OA: W4387640135

To better understand the stoichiometry of CD95L required to trigger apoptotic and nonapoptotic signals, we generated several CD95L concatemers from dimer to hexamer conjugated via a flexible link (GGGGS) 2 . These ligands reveal that although the hexameric structure is the best stoichiometry to trigger cell death, a dimer is sufficient to induce the apoptotic response in CD95‐sensitive Jurkat cells. Interestingly, only trimeric and hexameric forms can implement a potent Ca 2+ response, suggesting that while CD95 aggregation controls the implementation of the apoptotic signal, both aggregation and conformation are required to implement the Ca 2+ pathway.