Anti Inflammatory Activity Of Chitosan Nanoparticles With Chlorhexidine- An Invitro Study

Anjali Sankar , Sindhu Ramesh , S. Rajeshkumar , Nishitha Arun ·

YOU? · · 2023 · Open Access · · DOI: https://doi.org/10.47750/jptcp.2023.30.14.004

Introduction: One of the primary goals of root canal therapy is to relieve the discomfort associated with permanently injured pulps.Root canal therapy is the sole option for saving a tooth with irreversible pulpitis.Nonsteroidal anti-inflammatory medications can be used to treat inflammation (NSAIDs).All NSAIDs have the potential to induce severe adverse effects such as stomach ulcers, gastrointestinal bleeding, renal failure, heart attacks, and strokes.The aim of this study is to evaluate the anti-inflammatory activity of chitosan nanoparticles with chlorhexidine.Materials and Methods: The chitosan was obtained and plain chitosan with chlorhexidine, chitosan nanoparticles with chlorhexidine was prepared.The anti-inflammatory activity for Chitosan nanoparticles was tested by the following convention proposed by Muzushima and Kabayashi.Diclofenac Sodium was used as the standard.DMSO is used as a control.Percentage of protein denaturation was determined.Results: Anti-inflammatory activity of plain chitosan and nano chitosan showed an incremental pattern with increase in concentration, where the percentage of inhibition is high at 50μl.Its activity increases with increase in dosage.Nanochitosan with chlorhexidine shows higher antiinflammatory activity when compared to plain chitosan. Conclusion:The use of this novel irrigant in the field of endodontics would reduce the postoperative pain, the use of NSAID's and overall improve the experience of the patients undergoing endodontic treatment in the future.