Summary of different reporter protein expression kinetics. Article Swipe
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· 2016
· Open Access
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· DOI: https://doi.org/10.1371/journal.ppat.1005824.g006
· OA: W2508578250
The plot shows when a mCherry-cro signal is first detected relative to the time when a factory is first detected in that cell (tf). Each imaging experiment was repeated 3 times, and 4/10 fields in each experiment were analyzed in detail, to produce the 12 data points per infection that are shown here. The data show that the appearance of a mCherry-cro signal is significantly (****, p <0.001) delayed in cells co-infected with pE/L-mCherry(t) and mCherry-cro viruses, compared to any other kind of infection. Note that pE/L-mCherry(dup) infections show two patterns of gene expression, some virus produce an mCherry signal shortly after factories are detected, and others produce a signal delayed by ~3 h. The VACV-pE/L-mCherry(t) + pmCherry-cro experiment refers to cells where a promoterless mCherry-cro plasmid was transfected into cells infected with VACV-pE/L-mCherry(t). Note that only a single experiment was used to produce the 12 data points collected for the I1L-mCherry and A5-YFP infections, these serve as timing reference points for post-replicative and late VACV genes, respectively.
