bioRxiv (Cold Spring Harbor Laboratory)
Dietary Modulation of The Human Small Intestine Metabolome and Microbiome
2025
ABSTRACT The human gastrointestinal tract (GIT) is central to human physiology, yet our understanding of the metabolite, microbial, and hormonal spatiotemporal dynamics in humans is limited. Here, we employ naso-enteral intubation to generate a spatiotemporally resolved, multimodal dataset spanning the oral cavity (salivary fluid sampling), stomach, duodenum, and ileum of ten healthy participants. In a randomised trial, participants consumed legume-based meals prepared with varying degrees of structural deconstruc…