bioRxiv (Cold Spring Harbor Laboratory)
A yeast mating-based platform enables the generation and screening of ultra large antibody libraries
2025
Yeast display is a powerful platform for antibody engineering, offering eukaryotic protein folding and compatibility with fluorescence-activated sorting (FACS). However, conventional yeast display faces limitations in terms of library size and display heterogeneity due to plasmid-based expression. Here, we present a yeast display platform that combines genomic library integration and high-efficiency mating to generate ultra-diverse antibody libraries approaching the diversity of phage-libraries (>10 11 ). We en…