Salting Out
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Salting out (also known as salt-induced precipitation , salt fractionation , anti-solvent crystallization , precipitation crystallization , or drowning out ) is a purification technique that utilizes the reduced solubility of certain molecules in a solution of very high ionic strength. Salting out is typically used to precipitate large biomolecules, such as proteins or DNA. Because the salt concentration needed for a given protein to precipitate out of the solution differs from protein to protein, a specific salt concentration can be used to precipitate a target protein. This process is also used to concentrate dilute solutions of proteins. Dialysis can be used to remove the salt if needed.
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- Slug: salting-out
- Added: Jul 20, 2024